Linkage data and you may QTL mapping into the an effective tetraploid russet mapping people off potato

Linkage data and you may QTL mapping into the an effective tetraploid russet mapping people off potato

Genome-wider unmarried nucleotide polymorphism (SNP) markers combined with allele dose recommendations possess emerged since the a robust product to possess studying cutting-edge attributes inside expanded autotetraploid potato (Solanum tuberosum L., 2n = 4? = 48) additional reading. To date, this approach has been efficiently placed on the latest personality off quantitative characteristic loci (QTLs) hidden extremely heritable qualities for example state opposition, but largely unexplored for faculties having state-of-the-art patterns out-of heredity.

Overall performance

In this study, an F1 tetraploid russet mapping population (162 individuals) was evaluated for multiple quantitative traits over two years and two locations to identify QTLs associated with tuber sugar concentration, processing quality, vine maturity, and other high-value agronomic traits. We report the linkage maps for the 12 potato chromosomes and the QTL location with corresponding genetic models and candidate SNPs explaining the highest phenotypic variation for tuber quality and maturity related traits. Significant QTLs for tuber glucose concentration and tuber fry color were detected on chromosomes 4, 5, 6, 10, and 11. Collectively, these QTLs explained between 24 and 46% of the total phenotypic variation for tuber glucose and fry color, respectively. The QTL on chromosome 10 was associated with apoplastic invertases, with ‘Premier Russet’ contributing the favorable allele for fry processing quality. On chromosome 5, minor-effect QTLs for tuber glucose concentration and fry color co-localized with various major-effect QTLs, including vine maturity, growth habit, tuber shape, early blight (Altenaria tenuis), and Verticillium wilt (Verticillium spp.).

Conclusions

Linkage study and you can QTL mapping for the a russet mapping populace (A05141) playing with SNP dosage information properly identified beneficial alleles and you can candidate SNPs to own resistance to the accumulation out of tuber reducing sugar. These novel markers possess a high prospect of the advance from tuber operating top quality. Additionally, the newest advancement various genetic patterns to possess characteristics having overlapping QTLs from the readiness locus demonstrably suggests another genetic manage.

History

That have a complicated polyploid genetics the new expanded autotetraploid potato (Solanum tuberosum L., 2n = 4? = 48) gift ideas novel pressures for linkage mapping and you may decimal characteristic loci (QTL) research. The latest recent development of potato solitary nucleotide polymorphism (SNP) arrays provides some genome-broad indicators which you can use within the mapping and you will decimal attribute locus (QTL) analysis [1,2,step three,4]bined for the supply of mathematical equipment that allow the use off allele serving suggestions (we.age., the amount of copies of each and every allele during the confirmed polymorphic locus), it’s now you can easily so you’re able to rather improve strength out-of SNP pointers so you can detect recombination incidents in order to make use of this short article towards the QTL mapping [5,6,7]. Up to now, this process might have been properly always discover novel QTLs and you can candidate SNPs of this late blight resistance [cuatro, 8], inner temperature necrosis , Potato malware Y , and agronomic traits , however, mainly unexplored having understanding operating high quality or other higher-value qualities having cutting-edge heredity.

There’s a significance of molecular indicators linked to tuber operating top quality attributes to aid the selection of germplasm having cultivar invention. Control quality is actually determined by tuber glucose stuff. The fresh accumulation away from fructose and you will sugar (reducing sugars) from inside the potato tubers during cold storage negatively affects the conclusion-play with quality features. Cutting glucose posts try an intricate characteristic controlled by several hereditary and you can environmental things [12, 13]. The fresh cutting-edge hereditary tissues regarding the trait was similar to the craft of a lot minerals active in the metabolic pathways linking starch malfunction and you will synthesis to glucose creation and you can use [14, 15]. Hereditary mapping research shows you to definitely fry running high quality and reducing glucose was associated with 1000s of brief effect QTLs [sixteen,17,18,19,20]. Soltis-Kalina et al. identified a couple significant QTLs for chip colour to the chromosomes step 1 and 6 in the good diploid mapping society. Recently, Braun et al. said several QTLs getting resistance to cool-induced sweetening for the chromosomes cuatro and you can 6 out-of diploid potato. Many of these studies have already been did for the diploid potato germplasm. Although not, further research is necessary to most useful understand the hereditary buildings regarding these characteristics from the tetraploid height during the populations produced by russet-skinned potato varieties.

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